Part:BBa_K3219000:Design

dCas9-GFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1837
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 4116
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 644
  

Design Notes

We added linker 2A, which is a self-cleaving linker so that the dCas9 and GFP will fold independently.

Source

The dCas9 is from the Streptococcus pyogenes Type II CRISPR/Cas system. The GFP is from Aequoria victoria.